Publications
Cold exposure reinstates NAD+ levels and attenuates hepatocellular carcinoma
December 19, 2024
INTRODUCTION Hepatocellular carcinoma (HCC) is the most common type of primary malignant tumor that arises from hepatocytes, the main functional cells of the liver 1. It is a leading cause
Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
November 28, 2024
INTRODUCTION Cells are impacted by complex and co-occurring stresses throughout a life-course. Our goal was to generate physiologically relevant models of acute cellular stress, initially in relation to 3’ untranslated
Neuroglobin-enriched secretome provides neuroprotection against hydrogen peroxide and mitochondrial toxin-induced cellular stress
November 20, 2024
INTRODUCTION Cellular response to stress relies on evolutionary conserved mechanisms aimed at maintaining homeostasis and facilitating adaptation to new cellular environments 1. Under stressful conditions, post-mitotic neurons are hardly replaced.
Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure
October 17, 2024
INTRODUCTION Cellular stress is an inherent and inevitable aspect of cell physiology, prompting cells to adapt to environmental changes. Among the distinctive intracellular adaptive responses that have been characterized in
Dynamics of cell membrane lesions and adaptive conductance under the electrical stress
August 9, 2024
Exceeding physiological limits of the cell membrane potential compromises structural integrity, enabling the passage of normally impermeant solutes and disrupting cell function. Electropermeabilization has been studied extensively at the cellular scale, but not at the individual membrane lesion level. We employed fast total internal reflection fluorescence (TIRF) imaging of Ca2+ entry transients to discern individual lesions in a hyperpolarized cell membrane and characterize their focality, thresholds, electrical conductance, and the lifecycle. A diffuse and momentary membrane permeabilization without a distinct pore formation was observed already at a -100 mV threshold. Polarizing down to -200 mV created focal pores with a low 50- to 300-pS conductance, which disappeared instantly once the hyperpolarization was removed. Charging to - 240 mV created high-conductance (> 1 nS) pores which persisted for seconds even at zero membrane potential. With (...)
Saliva, a molecular reflection of the human body? Implications for diagnosis and treatment
May 27, 2024
INTRODUCTION The possibility of using biological fluids such as blood, urine, saliva and other biofluids to diagnose pathologies is a relatively old idea that has taken on a new lease
CircRNA regulates the liquid-liquid phase separation of ATG4B, a novel strategy to inhibit cancer metastasis?
May 24, 2024
Metastasis is the leading cause of death in cancer patients. Currently, there are no effective treatments to prevent cancer metastases. Therefore, it is imperative to provide more effective biomarkers and
Pathogenic hyperactivation of mTORC1 by cytoplasmic EP300 in Hutchinson-Gilford progeria syndrome
April 30, 2024
Mechanistic target of rapamycin complex 1 (mTORC1) is a multiprotein complex that serves as a pivotal regulator of cell growth, protein synthesis, metabolism and autophagy [1][2]. This complex compromises mTOR
The missing hallmark of health: psychosocial adaptation
March 12, 2024
INTRODUCTION The comprehension of health –defined in a positive fashion rather than as the absence of disease–requires a theory. We recently launched the bases of such a health theory by
MiR-200c reprograms fibroblasts to recapitulate the phenotype of CAFs in breast cancer progression
March 11, 2024
Mesenchymal-epithelial plasticity driving cancer progression in cancer-associated fibroblasts (CAFs) is undetermined. This work identifies a subgroup of CAFs in human breast cancer exhibiting mesenchymal-to-epithelial transition (MET) or epithelial-like profile with high miR-200c expression. MiR-200c overexpression in fibroblasts is sufficient to drive breast cancer aggressiveness. Oxidative stress in the tumor microenvironment induces miR-200c by DNA demethylation. Proteomics, RNA-seq and functional analyses reveal that miR-200c is a novel positive regulator of NFκB-HIF signaling via COMMD1 downregulation and stimulates pro-tumorigenic inflammation and glycolysis. Reprogramming fibroblasts toward MET via miR-200c reduces stemness and induces (...)