Volume 8 (2024)
Cold exposure reinstates NAD+ levels and attenuates hepatocellular carcinoma
Tatiana P Grazioso1,2,3, Maria del Mar Rigual1, Cristian Perna4,5, Eduardo J Caleiras6 and Nabil Djouder1
INTRODUCTION Hepatocellular carcinoma (HCC) is the most common type of primary malignant tumor that arises from hepatocytes, the main functional cells of the liver 1. It is a leading cause
Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
Dongyang Li1,2, Karina Al-Dahleh1,2, Daniel A Murphy2,3, Sonya Georgieva1, Nik Matthews4 and Claire L Shovlin1,2,5,*
INTRODUCTION Cells are impacted by complex and co-occurring stresses throughout a life-course. Our goal was to generate physiologically relevant models of acute cellular stress, initially in relation to 3’ untranslated
Neuroglobin-enriched secretome provides neuroprotection against hydrogen peroxide and mitochondrial toxin-induced cellular stress
Giovanna Bastari1,a, Virginia Solar Fernandez1,2,a, Maurizio Muzzi3,4, Sandra Moreno3,5, Maria Marino1,5 and Marco Fiocchetti1,5
INTRODUCTION Cellular response to stress relies on evolutionary conserved mechanisms aimed at maintaining homeostasis and facilitating adaptation to new cellular environments 1. Under stressful conditions, post-mitotic neurons are hardly replaced.
Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure
Hebatallah Abdelrasol1,a, Avika Chopra1,a, Liana Shvachiy1, Dirk Beutner2, Tiago F Outeiro1,3,4,5,* and Cristian Setz2,1,*
INTRODUCTION Cellular stress is an inherent and inevitable aspect of cell physiology, prompting cells to adapt to environmental changes. Among the distinctive intracellular adaptive responses that have been characterized in
Dynamics of cell membrane lesions and adaptive conductance under the electrical stress
Mantas Silkunas, Olga N Pakhomova, Giedre Silkuniene and Andrei G. Pakhomov
Exceeding physiological limits of the cell membrane potential compromises structural integrity, enabling the passage of normally impermeant solutes and disrupting cell function. Electropermeabilization has been studied extensively at the cellular scale, but not at the individual membrane lesion level. We employed fast total internal reflection fluorescence (TIRF) imaging of Ca2+ entry transients to discern individual lesions in a hyperpolarized cell membrane and characterize their focality, thresholds, electrical conductance, and the lifecycle. A diffuse and momentary membrane permeabilization without a distinct pore formation was observed already at a -100 mV threshold. Polarizing down to -200 mV created focal pores with a low 50- to 300-pS conductance, which disappeared instantly once the hyperpolarization was removed. Charging to - 240 mV created high-conductance (> 1 nS) pores which persisted for seconds even at zero membrane potential. With (...)
Saliva, a molecular reflection of the human body? Implications for diagnosis and treatment
Vincent Géli and Norbert Nabet
INTRODUCTION The possibility of using biological fluids such as blood, urine, saliva and other biofluids to diagnose pathologies is a relatively old idea that has taken on a new lease
CircRNA regulates the liquid-liquid phase separation of ATG4B, a novel strategy to inhibit cancer metastasis?
Ziyuan Guo1,#, Yang Chen1,#, Yaran Wu1 and Jiqin Lian1
Metastasis is the leading cause of death in cancer patients. Currently, there are no effective treatments to prevent cancer metastases. Therefore, it is imperative to provide more effective biomarkers and
Pathogenic hyperactivation of mTORC1 by cytoplasmic EP300 in Hutchinson-Gilford progeria syndrome
Lucille Ferret1,2,3, Guido Kroemer1,2,4 and Mojgan Djavaheri-Mergny1,2
Mechanistic target of rapamycin complex 1 (mTORC1) is a multiprotein complex that serves as a pivotal regulator of cell growth, protein synthesis, metabolism and autophagy [1][2]. This complex compromises mTOR
The missing hallmark of health: psychosocial adaptation
Carlos López-Otín1,2,3 and Guido Kroemer1,4,5
INTRODUCTION The comprehension of health –defined in a positive fashion rather than as the absence of disease–requires a theory. We recently launched the bases of such a health theory by
Cold exposure reinstates NAD+ levels and attenuates hepatocellular carcinoma
Tatiana P Grazioso1,2,3, Maria del Mar Rigual1, Cristian Perna4,5, Eduardo J Caleiras6 and Nabil Djouder1
INTRODUCTION Hepatocellular carcinoma (HCC) is the most common type of primary malignant tumor that arises from hepatocytes, the main functional cells of the liver 1. It is a leading cause
Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
Dongyang Li1,2, Karina Al-Dahleh1,2, Daniel A Murphy2,3, Sonya Georgieva1, Nik Matthews4 and Claire L Shovlin1,2,5,*
INTRODUCTION Cells are impacted by complex and co-occurring stresses throughout a life-course. Our goal was to generate physiologically relevant models of acute cellular stress, initially in relation to 3’ untranslated
Neuroglobin-enriched secretome provides neuroprotection against hydrogen peroxide and mitochondrial toxin-induced cellular stress
Giovanna Bastari1,a, Virginia Solar Fernandez1,2,a, Maurizio Muzzi3,4, Sandra Moreno3,5, Maria Marino1,5 and Marco Fiocchetti1,5
INTRODUCTION Cellular response to stress relies on evolutionary conserved mechanisms aimed at maintaining homeostasis and facilitating adaptation to new cellular environments 1. Under stressful conditions, post-mitotic neurons are hardly replaced.
Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure
Hebatallah Abdelrasol1,a, Avika Chopra1,a, Liana Shvachiy1, Dirk Beutner2, Tiago F Outeiro1,3,4,5,* and Cristian Setz2,1,*
INTRODUCTION Cellular stress is an inherent and inevitable aspect of cell physiology, prompting cells to adapt to environmental changes. Among the distinctive intracellular adaptive responses that have been characterized in
Dynamics of cell membrane lesions and adaptive conductance under the electrical stress
Mantas Silkunas, Olga N Pakhomova, Giedre Silkuniene and Andrei G. Pakhomov
Exceeding physiological limits of the cell membrane potential compromises structural integrity, enabling the passage of normally impermeant solutes and disrupting cell function. Electropermeabilization has been studied extensively at the cellular scale, but not at the individual membrane lesion level. We employed fast total internal reflection fluorescence (TIRF) imaging of Ca2+ entry transients to discern individual lesions in a hyperpolarized cell membrane and characterize their focality, thresholds, electrical conductance, and the lifecycle. A diffuse and momentary membrane permeabilization without a distinct pore formation was observed already at a -100 mV threshold. Polarizing down to -200 mV created focal pores with a low 50- to 300-pS conductance, which disappeared instantly once the hyperpolarization was removed. Charging to - 240 mV created high-conductance (> 1 nS) pores which persisted for seconds even at zero membrane potential. With (...)
Saliva, a molecular reflection of the human body? Implications for diagnosis and treatment
Vincent Géli and Norbert Nabet
INTRODUCTION The possibility of using biological fluids such as blood, urine, saliva and other biofluids to diagnose pathologies is a relatively old idea that has taken on a new lease
Saliva, a molecular reflection of the human body? Implications for diagnosis and treatment
Vincent Géli and Norbert Nabet
INTRODUCTION The possibility of using biological fluids such as blood, urine, saliva and other biofluids to diagnose pathologies is a relatively old idea that has taken on a new lease
CircRNA regulates the liquid-liquid phase separation of ATG4B, a novel strategy to inhibit cancer metastasis?
Ziyuan Guo1,#, Yang Chen1,#, Yaran Wu1 and Jiqin Lian1
Metastasis is the leading cause of death in cancer patients. Currently, there are no effective treatments to prevent cancer metastases. Therefore, it is imperative to provide more effective biomarkers and
Pathogenic hyperactivation of mTORC1 by cytoplasmic EP300 in Hutchinson-Gilford progeria syndrome
Lucille Ferret1,2,3, Guido Kroemer1,2,4 and Mojgan Djavaheri-Mergny1,2
Mechanistic target of rapamycin complex 1 (mTORC1) is a multiprotein complex that serves as a pivotal regulator of cell growth, protein synthesis, metabolism and autophagy [1][2]. This complex compromises mTOR
The missing hallmark of health: psychosocial adaptation
Carlos López-Otín1,2,3 and Guido Kroemer1,4,5
INTRODUCTION The comprehension of health –defined in a positive fashion rather than as the absence of disease–requires a theory. We recently launched the bases of such a health theory by