Putting together and taking apart: assembly and disassembly of the Rad51 nucleoprotein filament in DNA repair and genome stability

Authors:

Tadas Andriuskevicius1, Oleksii Kotenko1 and Svetlana Makovets1
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Affiliations:

1 Institute of Cell Biology, School of Biological Sciences, University of Edinburgh.

Keywords: 

homologous recombination, Rad51 filament, double-stranded DNA break, DNA repair, Rad51 regulation.

Abbreviations:

BIR – break-induced replication,

DNTA – de novo telomere addition,

DSB – double-stranded DNA break,

dsDNA – double-stranded DNA,

GC – gene conversion,

HR – homologous recombination,

PTM – post-translational modification,

RFC – Replication Factor C,

RPA –Replication Protein A,

SDSA – synthesis-dependent strand annealing,

SIM – SUMO-interacting motif,

SSA – single-stranded annealing,

ssDNA – single-stranded DNA.

Corresponding Author(s):

Svetlana Makovets, Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Alexander Crum Brown Road, King’s Buildings, Edinburgh, EH9 3FF, United Kingdom Sveta.Makovets@ed.ac.uk

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Tadas Andriuskevicius, Oleksii Kotenko and Svetlana Makovets (2018). Putting together and taking apart: assembly and disassembly of the Rad51 nucleoprotein filament in DNA repair and genome stability. Cell Stress 2(5): 96-112. doi: 10.15698/cst2018.05.134

© 2018 Andriuskevicius et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.

Abstract:

Homologous recombination is a key mechanism providing both genome stability and genetic diversity in all living organisms. Recombinases play a central role in this pathway: multiple protein subunits of Rad51 or its orthologues bind single-stranded DNA to form a nucleoprotein filament which is essential for initiating recombination events. Multiple factors are involved in the regulation of this step, both positively and negatively. In this review, we discuss Rad51 nucleoprotein assembly and disassembly, how it is regulated and what functional significance it has in genome maintenance.