Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 7: Shared differentially expressed genes in same-donor human peripheral blood mononuclear cells (PBMCs) following ‘treatments’ by either Wash preparation and 1h control treatment, or plasma-resuspension and 1h cycloheximide treatment. (A) Wash method 1 (Ficoll-prepared) PBMCs compared to same-donor, plasma-resuspended PBMCs (donors 4-6). (B) Wash method 2 (CPTTM)-prepared PBMCs compared to same-donor, plasma-resuspended PBMCs (donors 7-9). i) Venn diagrams of genes differentially expressed in each setting, highlighting genes differentially expressed in both settings. ii) Heat maps of log2 (fold change) in gene expression for the 271 genes (Donors 4-6) or 358 genes (Donors 7-9) that were differentially expressed in PBMCs across both treatments. iii) KEGG pathways [53–55] identified by the 271 genes (Donors 4-6) or 358 genes (Donors 7-9). These identical representations resulted from differential expression of different genes for each term as shown in Supplementary Table S8.