Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 6: Differential gene expression in same-donor human peripheral blood mononuclear cells (PBMCs) prepared by Wash Method 2 after 1h cycloheximide treatment. (A) Wash method 2 (CPTTM)-prepared PBMCs compared to same-donor, plasma-resuspended PBMCs (donors 7-9). Note similarity with subset of genes in Donors 1-3 (Fig. 2B). (B) KEGG pathways [53–55] identified by genes differentially expressed after 1h cycloheximide 100µg/mL in i) plasma-resuspended PBMCs and ii) same-donor Wash Method 2-prepared PBMCs.