Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 5: Differential gene expression in same-donor human peripheral blood mononuclear cells (PBMCs) prepared by wash method 1 after 1h cycloheximide treatment. (A) Wash method 1 (Ficoll-prepared) PBMCs compared to same-donor, plasma-resuspended PBMCs (donors 4-6). (B) KEGG pathways [53–55] identified by genes differentially expressed after 1h cycloheximide 100µg/mL in i ) plasma-resuspended PBMCs and ii) same-donor Ficoll-prepared PBMCs (Wash Method 1).