FIGURE 4: Cold-induced acetyl-CoA carboxylase inhibition. (A) NAD⁺ levels in 21-week-old hURI^Hep mouse livers exposed to RT or CT. (B) Scheme of U2O2 cells treated undernormal temperature (37^◦C) or cold temperature (27^◦C). (C) Western blot analysis of U2OS cells exposed for 24 hours to 27^◦C or 37^◦C. (D-E) Representative pictures of IF staining’s in U2OS cells incubated at 27^◦C or 37^◦C for 24 hours. (F) Western blot analysis of U2OS cells incubated at 27^◦C or 37^◦C for 24 hours.