Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 8: Volcano plots of genes differentially expressed in human peripheral blood mononuclear cells (PBMCs) after 1h treatment with 40 µmol/L ferric citrate. (A) Volcano plots of genes differential expressed in PBMCs from donors 4-6: i) resuspended in plasma, or ii) prepared using Wash Method 1. (B) Volcano plots of genes differential expressed in PBMCs from donors 7-9: i) resuspended in plasma, or ii) prepared using Wash Method 2. Note that genes differentially expressed to p<10−5were all in the washed, media-treated PBMCs where IRF4, ICOS1, and NR4A3 were the most differentially expressed genes (Aii) for donors 4-6 PBMCs after Wash 1, and (Bii) for donors 7-9 PBMCs after Wash 2. IRF4 encodes interferon regulatory factor 4; ICOS1, inducible T cell costimulator, and NR4A3 encodes a steroid-thyroid hormone-retinoid receptor transcriptional activator.