Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 4: Basal differences in same-donor human peripheral blood mononuclear cells (PBMCs) prepared using CPTTM tubes with and without washes. (A) Volcano plot of the 2,205 genes differentially expressed in PBMCs from Donors 7-9 that were isolated using CPTTM tubes, with washes then resuspension in FCS-supplemented RPMI media, compared to simultaneously-prepared PBMCs from the same donors maintained in endogenous plasma. (B) KEGG pathways [53–55] identified by the differentially expressed genes. (C) Heat map of log2 (fold change) in gene expression for the 1,378 genes that were differentially expressed in PBMCs across both sets of donor trios following washes, compared to plasma resuspended PBMCs from the same donor. As shown in Supplementary Table 5B, the 1,378 genes were those that were differentially expressed for both Wash 1 (coloured symbols in Figure 3A) and Wash 2 (coloured symbols in Figure 4A). Note marked similarities in these genes differentially expressed by the different wash protocols in the different donor trios.