Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 3: Basal differences in same-donor human peripheral blood mononuclear cells (PBMCs) prepared by Wash Method 1 compared to plasma resuspension. (A) Volcano plot of the 2,388 genes differentially expressed to p<0.05 in Ficoll-prepared PBMCs from Donors 4-6 that were washed and resuspended in serum-supplemented RPMI media (Wash Method 1), compared to simultaneously-prepared PBMCs from the same donors that were not washed, and were maintained in endogenous plasma throughout preparation, treatment and final centrifugation steps. Thresholds for the volcano plot of genes differentially expressed to p<0.05 were log2(fold change) >1(red) and <1 (blue). (B) KEGG (Kyoto Encyclopaedia of Genes and Genomes) [53–55] pathways identified by the differentially expressed genes.