Back to article: Endogenous plasma resuspension of peripheral blood mononuclear cells prevents preparative-associated stress that modifies polyA-enriched RNA responses to subsequent acute stressors
FIGURE 2: Differential gene expression in human peripheral blood mononuclear cells (PBMCs) after 1h stress treatments. (A) Raw alignments in polyA-selected RNASeq libraries generated from PBMCs from donors 1-3 after 1hr in control and experimental treatments. 32◦C was the temperature used to model mild/moderate hypothermia; Fe, 10 µmol/L ferric citrate to provide a mild oxidative stress; CHX, 100 µg/mL cycloheximide to inhibit protein translation and model the common output of the integrated stress response. TNF encodes TNFα [7]; IL1B encodes interleukin-1β [46]; FOS encodes c-FOS [47]; ATF3 encodes activating transcription factor (ATF)3 [49]. Mean, standard deviation and individual values (N=3, from donors 1-3) are displayed. (B) Volcano plot of genes differentially expressed after 1h cycloheximide. Thresholds for the volcano plot of genes differentially expressed to adjusted p<0.05 were log2(fold change) >1 (red) and <1 (blue).