FIGURE 8: RNA is present in SGs formed in response to cisplatin treatment. (A) Electropherogram obtained with the Agilent Bioanalyzer 2100 analysis for total RNA extracted from cisplatin-treated H4 cells after SG enrichment showing the ladder at 22.50 nt length, short peaks at 26.52 nt and 27.25 nt corresponding to 5S and 5.8S rRNA, as well as further peaks at 42.50 nt and 49.85 nt length corresponding to 18S and 28S rRNA. (B) Electropherogram obtained with the Agilent Bioanalyzer 2100 analysis for negative control: total RNA treated with RNase. (C) Electropherogram obtained with the Agilent Bioanalyzer 2100 analysis after rRNA depletion. (D) Bands representation of electropherograms obtained with the Agilent Bioanalyzer 2100 analysis for total RNA extracted from SG-enriched cisplatin-treated H4 cells. The first lane shows the ladder with RNA bands at 25, 200, 500, 1000, 2000, and 4000 nucleotides. The second lane corresponds to total RNA with bands for rRNA 5S (26.52 nt), 18S (42.50 nt), and 28S (49.85 nt). The third lane shows RNA bands after degradation with RNase ATM . The fourth lane shows the RNA bands remaining after rRNA depletion.