Back to article: Stress granules formation in HEI-OC1 auditory cells and in H4 human neuroglioma cells secondary to cisplatin exposure
FIGURE 7: Enrichment of SG cores from cells treated with cisplatin. (A) Schematic representation of the protocol for SG core enrichment adapted from Wheeler et al., 2017 [27] . (B) Western blot for G3BP1 in arsenite and cisplatin-treated H4 cell lysate (T) and their SG cores enriched fraction (EF). (C) Representative image of total protein amounts in T and EF from both arsenite and cisplatin-treated H4 cells. The relative amounts of total protein are shown at the bottom of each lane (D) Log2 plot of fold enrichment change in G3BP1 in EF relative to T.