Back to article: p38β MAPK mediates ULK1-dependent induction of autophagy in skeletal muscle of tumor-bearing mice
FIGURE 5: p38β MAPK is required for upregulation of C/EBPβ-responsive autophagy-related genes in cachectic muscle of tumor-bearing mice. (A) Inhibition of p38 MAPK attenuates LC3b and Gabarapl1 upregulation in cachectic muscle of LLC tumor-bearing mice. On day 7 of LLC cells implant to mice, SB202190 was i.p. injected (5 mg/kg) daily with DMSO (50%) as vehicle control. Lysate of TA collected on day 21 was analyzed by qPCR for LC3b and Gabarapl1 mRNA levels. (B) LC3b and Gabarapl1 upregulation in the muscle of LLC tumor-bearing mice is dependent on p38β MAPK. Mice with muscle-specific knockout of p38β MAPK (p38β MAPK MKO) or control mice (p38β MAPKf/f) were implanted with LLC cells. On day 21, total RNA was extracted from TA of the mice and analyzed by qPCR for LC3b and Gabarapl1 mRNA levels. Data were analyzed by one way ANOVA. * denotes a difference (p < 0.05).