FIGURE 3: GLT inhibits canonical and non-canonical TGFβ signaling. CS-99 and UMMT-ARK1 cells were grown on a thin layer of Matrigel and treated with TGFβ1, GLT and Cisplatin (CIS, 2.5 µM) as represented, post 24 h of treatment, cell lysate was immunoblotted for (A) canonical and non-canonical markers of TGFβ1 signaling proteins along with Cyclin D1 as a marker for cell cycle progression and Tubulin as the loading control. (B) Effect of TGFβ1 and GLT + TGFβ1 treatment on the EMT markers (FBN – Fibronectin, NCAD – N-cadherin) were evaluated using immunoblotting, GAPDH was used as the loading control.