FIGURE 1: In vivo multiphoton microscopy images of mitochondrial Ca²⁺ in neurons in the Wt and APP/PS1 mice. Mice were injected with AAV.hSyn.2mtYC3.6 and a cranial window was implanted. Mitochondrial Ca²⁺ was later evaluated with multiphoton microscopy. Pictures are representative of old wild-type (Wt, top) and transgenic mice (APP/PS1 Tg, bottom). The field of view shows the AAV transduction in neuronal mitochondria (green) and the blood vessels labeled with fluorescent Dextran (red). Pseudocolor images represent the color coded mitochondrial Ca²⁺ concentrations for the corresponding field of view images. Arrow points to a neuron with mitochondrial Ca²⁺ overload. Scale bar represents 20 μm.