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FIGURE 4: Dose dependent inhibition of the migration in AuNPs treated pancreatic cancer cell lines. PANC-1 (a) and AsPC-1 (b) were first starved for 24 h after seeding, and then treated with AuNPs (5 μg/ml or 25 μg/ml) for 48 h under the starvation condition. After the treatment, cells were collected and seeded in the upper chamber of the transwell separately at the concentration of 1×10⁵/well; cell culture medium including 1% FBS was added into the lower chamber. 16 h later, the non-migrated cells were removed with a cotton swab, and the migrated cells were stained and quantified (c, d). Independent triplicate experiments were repeated. Values are means ± SD and statistical analysis were performed using one-way ANOVA. *P≤0.05, **P≤0.01.