FIGURE 5: Cac1 functions in a different pathway from that of Rtt101-Mms1-Mms22 in cohesion. Genetic interactions among RTT101, MMS1, MMS22, SCC1, and CAC1. (A) Effect associated with RTT101 inactivation on viability of cac1Δ, scc1-73, and cac1Δ scc1-73 cells. (B) Effect associated with MMS1 inactivation on viability of cac1Δ, scc1-73, and cac1Δ scc1-73 cells cells. (C) Effect associated with MMS22 inactivation on the viability of cac1Δ, scc1-73, and cac1Δ scc1-73 cells. Genetic interactions were assessed by spotting a tenfold series dilution of cells of the indicated genotype onto YPD. Cells were grown at 30° for 3 days (left), or at 25°C (right) for 4 days.