FIGURE 4: Essential sister chromatid cohesion genes are required for the viability of CAF-1 deficient cells. (A) Genetic interaction of CAC1 with ECO1. Left, fifty tetrads from eco1Δ/ECO1 cac1Δ/CAC1 rad61Δ/RAD61 diploid strain were dissected. Tetrads were grown at 30° for 5 days. Dashed circles indicate eco1Δ cac1Δ rad61Δ mutant. Right, top, tenfold serial dilutions of indicated genotypes were spotted onto YPD plates and incubated at 30° for 3 days. Right, down, tenfold serial dilutions of wild-type, cac1Δ, eco1-1, and eco1-1 cac1Δ cells were spotted onto YPD plates and incubated at 25° (left), or 36°C (right) for 3 days. (B) Genetic interaction of CAC1 with SCC2. Tenfold serial dilutions of exponentially growing cells were spotted onto YPD plates and incubated at 25° (left), or 32° (right) for 3 days. (C) Genetic interaction of CAC1 with SCC1. Tenfold serial dilutions of exponentially growing cells were spotted onto YPD plates and grown at 25° (left), or 32° (right) for 3 days.