FIGURE 8: Eco1-catalyzed Smc3 acetylation is reduced in the absence of Cac1. Cells of the indicated genotypes were synchronized in G1 using α-factor and released into nocodazole-imposed mitotic arrest at 25°. Top, FACS analysis of the DNA content was used to monitor cell cycle progression. Middle, acetylated Smc3 was immunoblotted in the cell lysate by an antibody specific to Smc3-K112,113Ac (gift from Adele Marston, The Wellcome Centre for Cell Biology, Edinburgh, UK). Down, quantitative Western blotting analyses of Smc3-K112,113 acetylation. The results represented the average of six independent experiments. Statistical significance was measured using Two Tailed Mann-Whitney test.