FIGURE 6: Cac1 is important to maintain cohesion in ctf4Δ cells. Sister chromatid cohesion was analyzed by monitoring the tagged centromere of chromosome III. Top, experimental design. DNA replication was monitored by FACS analysis of DNA content. Over 100 cells were counted for each experiment. The results represent the average of five independent experiments. Statistical significance was measured using Two Tailed Mann-Whitney test.