FIGURE 3: ZEA blocks cell cycle progression through targeting the potassium channels in the early stages of ZEA treatment. (A) Immunoblotting for KCNJ4, KCNJ12, PINK1 and actin expression after ZEA treatment as well as assessment of cell cycle progression in HCT-8. (B) Immunoblotting for KCNJ4, KCNJ12 and actin expression after ZEA treatment as well as assessment of cell cycle progression in overexpression cell lines. (C) Immunoblotting for KCNJ4, KCNJ12 and actin expression after ZEA treatment as well as their cell cycle in knockout cell lines. (D) ZEA toxin relative concentrations (extracellular and intracellular) in HCT-8 cells exposed to ZEA toxin. (E) Knockout and overexpression of PINK1 and the percentage cellular viability of cell lines after treatment with either 25 or 30 µM of ZEA. (F) Knockout and overexpression of PINK1 and assessment of cell cycle progression. Data are presented as the mean ± SEM (n=3). p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***), calculated by Student t-test.