Back to article: COX4-1 promotes mitochondrial supercomplex assembly and limits reactive oxide species production in radioresistant GBM
FIGURE 6: O2•− production is reduced in COX4-1 overexpressing GBM cells. Quantification of mitochondrial O2•− production in vector-transfected controls and COX4-1-overexpressing U251 cells (A) and D456 (B) cells treated with or without AA (10 μM for 15 min), assessed by MitoSOX assay. Data are presented as the mean ± SEM (n= 3). Clonogenic survival curves for vector-transfected controls and COX4-1-overexpressing U251 (C) and D456 (D) cells. Cells were irradiated with 2, 4, 6, or 8 Gy and immediately plated. Clonogenic survival was estimated on day 14 after irradiation. p < 0.01 (**) and p < 0.001 (***), calculated using one-way ANOVA followed by Tukey's multiple comparison test. p < 0.0001 (****), calculated by Student t-test. a.u., arbitrary units; AA, antimycin A; ns, not significant.