FIGURE 5: AuNPs decrease IC₅₀ of gemcitabine on pancreatic cancer cell lines. After seeding, cells were first treated with/without AuNPs (25 μg/ml) for 24 h in the complete medium, followed by gemcitabine for 72 h. The proliferation of PANC-1 (a), AsPC-1 (b), MIA-PaCa-2 (c) and HPAF-II (d) were measured with MTT and the IC₅₀ were calculated with Prism. The IC₅₀ value are shown in the table (e). The proliferation of each cell line at the concentration of 10 nM of gemcitabine was calculated (f). Independent triplicate experiments were repeated and each time, at least triplicate wells were used. Values are means ±SD and statistical analysis were performed using one-way ANOVA.