FIGURE: 3 Neuronal knockdown of Lamin causes increased failure and latency of the DLM. (A) Schematic of experimental setup for recording from the GFS. TTM = tergotrochanteral muscle; TTMn = tergotrochanteral motorneuron; PSI= peripherally synapsing interneuron; DLM = dorsal longitudinal muscle; DLMNn = dorsal longitudinal motorneuron. Representative traces from the DLM in (B) elav/+ and (C) Lamin knockdown flies (elav/Lam IR) aged 50 days, stimulated at 150 Hz. Asterisks indicate failure to respond. Mean number of failures for elav/+ and Lamin knockdown flies (elav/Lam IR) at (D) 10 days, (E) 30 days and (F) 50 days old. (G-I) The probability of DLM response in Lamin knockdown flies (elav/Lam IR) is significantly decreased after repeated stimulations, in an age-dependent manner. (J) No significant difference in latency was found in young flies (10 days) with Lamin knockdown (elav/Lam IR) when compared to elav/+. (K-L) Aged flies (30 days and 50 days) show a significant increase in DLM latency with Lamin knockdown (elav/Lam IR). (M-O) The probability of TTM response in Lamin knockdown flies (elav/Lam IR) shows no significant difference from elav/+. n³7 animals per group. Data are mean ± SEM. Data was analysed using two-way ANOVA with Bonferroni test for multiple comparisons. Response latency was analysed using multiple t-tests with Holdam-Sidak correction for multiple comparisons. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. elav/+ = elavGal4; +/+; UAS-mCD8GFP > w¹¹¹⁸; elav/Lam IR = elavGal4; +/+; UAS-mCD8GFP > Lam IR.