FIGURE 7: Effect of miR-200c in fibroblasts on resistance to chemo-immuno-therapy. (A) Representative PD-L1 staining in tumor sections. Co-injection of 4T1 cells and miR-200c NIH3T3 fibroblasts or controls (scale bar: 20μm). Immunohistochemistry quantification for PD-L1 in random units as per ImageJ quantification. (B-D) Co-injection with AT3 cells and MEFs miR-200c or control. (B) PD-L1 immunoblot. (C, D) Tumor growth with chemo-immunotherapy. Mice were treated by IP with Paclitaxel and anti-PD-L1 versus DMSO and control IgG after tumor implantation.