FIGURE 2: Effects of carcinoma cells and miR-200c on fibroblasts. (A) CD24 and CD44 expression patterns by flow cytometry in BJ1 fibroblasts under homotypic and co-culture conditions with MCF7 carcinoma cells. (B) Fold change of miR-200c and miR-205 expression in BJ1 fibroblast cells co-cultured with carcinoma cells compared to homotypic culture. (C) Flow plots showed CD24 and CD44 level change in BJ1 cells overexpressing miR-200c and scramble control. (D) Images of cell morphology and K8/18 (Red) expression of BJ1 with precursor miR-200c expression and scramble control (scale bar: 20 μm). (E) Senescence β-galactosidase staining in BJ1 cells overexpressing miR-200c and scramble control. (F, G) Fold change in miR-200c and miR-205 expression in BJ1 and HS5 fibroblast cells with overexpression of miR-200c or control. *p<0.05.