FIGURE 2: Hallmarks of mRNA encoding MLKL induced cell death. Schematic representation of in vitro cell death of B16 cells transfected with MLKL-mRNA. Upper left: 24 hours after transfection, cells were collected and analyzed by flow cytometry. The graph shows a schematic flow cytometric plot of sytox positive cells. Upper right: Impact of the pan-caspase inhibitor zVAD-fmk on cell death induction of apoptotically dying cells or cells transfected with MLKL-mRNA. Lower right: Caspase 3 activity over time in apoptotically dying cells or cells transfected with MLKL-mRNA. Lower left: Still images of apoptotically dying cells or 24 h after transfection with MLKL-mRNA visualized by time-lapse microscopy.