Back to article: Neuronal Lamin regulates motor circuit integrity and controls motor function and lifespan
FIGURE 1: Neuronal knockdown of Lamin causes decreased lifespan and locomotor ability. (A) Pan neuronal knockdown of Lamin (elav/Lam IR) causes a significant decrease in lifespan compared to control flies (elav/+). (B) qPCR for knockdown efficiency of Lamin RNAi hairpins showed a significant decrease in Lamin mRNA expression for both hairpins. (C) Western blot of fly heads showed a significant decrease in Lamin protein expression with Lam IR-1 and Lam IR-2 knockdown. n=5. (D-E) Locomotor ability is significantly impaired in elav/Lam IR flies. n³50 animals per group. Data represents mean ± SEM. Climbing, western blot and qRT-PCR data was analysed using one-way ANOVA with post-hoc Dunnett test for multiple comparisons. Lifespan analysis was done using a log-rank (Mantel-Cox test). *comparison between elav/+ and elav/Lam IR-1. #comparison between elav/+ and elav/Lam IR-2. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. elav/+ = elavGal4; +/+; UAS-mCD8GFP > w1118; Lam IR/+ = w1118 > Lam IR; elav/Lam IR = elavGal4; +/+; UAS-mCD8GFP > Lam IR.