FIGURE 7: Methylation state of the H19 promoter in chemoresistant cells. (A) Analyzed H19 promoter region. The bisulphite-converted DNA sequence is shown; CpG sites 1-23 (Chr.11: 2,019,761 – 2,019,488) are la- belled in grey; amplification primers are underlined. (B, C) Absolute methylation of 23 CpG sites located in the H19 promoter in chemo-resistent (resistant) and chemosensitive (control) HepG2 (up- per panels), Plc/Prf/5 (middle panels), and Huh7 (bottom panels) cells analyzed by Bi-PROF (n=3, triplicates). (B) Doxorubicin resistant and (C) sorafenib resistant hepatoma cells. The p values were calculated by two-sample t-test or Mann-Whitney U test depending on the data distribution. * p < 0.05, ** p < 0.01, *** p < 0.001.