FIGURE 2: Transfection and selection of mNIS expressing KRT19 edited and control pancreatic cancer cells. (A and B) Schematic illustration of the workflow to obtain stable mNIS expression in transduced tumor cell lines without additional expression of potentially immunogenic in vitro selection markers. (C) Flow cytometry of expanded monoclonal sgKRT19 and sgScramble cancer cells prior to transduction (Ctrl), then before (Pre) and after (Post) adeno-Flpo transduction. (D) Western blot analysis for mNIS and KRT19 expression in monoclonal sgKRT19 and sgScramble cells following Ad-Flpo transfection and selection for the absence of GFP fluorescence (left two lanes). Blotting results on protein extracts from both non-mNIS expressing cells (NIS ctrl-) and mNIS expressing cells (NIS ctrl+) are shown in the right two lanes.