FIGURE 1: The immunostealth vector for mNIS-labeling of cells. A schematic illustration of the vector used to establish stable mNIS expression in tumor cells without potential immunogenicity arising from selection marker expression. Abbreviations; FRT – flippase recognition target. Flpo – flippase. PGK – phosphoglycerate kinase 1 promoter. TurboGFP – fast-maturing green fluorescent protein. T2A – self-cleaving 2A peptide. Hygro – hygromycin B phosphotransferase. EF1 – elongation factor-1 alpha promoter. mNIS – murine sodium iodide symporter (Slc5a5). Note; underlined transgenic sequence remains stably integrated in the transduced cell genome following Flpo recombination.